Biosecurity is widely acknowledged worldwide as a core measure for preventing the spread of infectious diseases at the farm level. This study surveyed 240 smallholder swine farmers across Tra Vinh and Long An provinces to examine their sociodemographic characteristics, biosecurity management (BM) practices, perception, and attitudes toward BM. It further assessed how socioeconomic and psychological factors influence BM status. Descriptive statistics and multiple linear regression analyses were explored to examine all the relationships. The results indicated that farmers were more inclined to plan, implement, and adjust biosecurity measures than to adjust biosecurity practices. Planning the biosecurity practices accounted for the highest proportion (91.70%), whereas adjustment was the lowest (65.00%). In terms of attitudes, 62.10% of respondents expressed understanding well in adopting BM, and only 40.40% believed that biosecurity management improved their swine production. The factors, including education, occupation, and perception, were found to significantly positively influence the adoption of biosecurity management practices. Overall, this finding implies that policymakers should provide training short courses, awareness programs, and practical demonstrations to strengthen confidence in biosecurity management and promote its effective and long-term application.