Anther culture technology is one of the most rapid methods that can be used to accelerate plant breeding programmes by producing double haploids. Therefore, this study aimed to develop an effective callus induction medium for selected tomato genotypes. Four treatments with varying Benzyl Amino Purine (BAP) concentrations (0, 1, 2, and 3 mg L-1) were tested, each containing 2 mg L-1 Kinetin and 1 mg L-1 Naphthalin Acetic Acid (NAA) on full-strength MS medium. Flower buds from varieties NO-312, HT 05, Bathiya, L-33, and 12-561 were used. After preparation and surface sterilization, the anthers were incubated at 25°C in the dark for 14 days to induce callus formation. Results showed that the highest callus formation (42.08±14.2%) was in the 0 mg L-1 BAP treatment for variety NO-312. The highest callus greening (14.28±14.2%) was in the 0 mg L-1 BAP treatment for variety HT 05. Thus, NO-312 was the best for callus formation and HT 05 for callus greening. The study indicates that the absence of BAP positively affects callus formation and greening in these tomato genotypes, aiding in refining anther culture methods for faster plant breeding